Alphinity
Transforming Bioprocessing with PIXER^®

Biomanufacturing demands precision at every turn—whether safeguarding sensitive biologics like monoclonal antibodies and live cells or ensuring accurate flow rates and overall system efficiency. Bioprocessing pumps are at the heart of this operation, entrusted with delivering fluids steadily and consistently. Yet, traditional pumps—hampered by pulsation, shear stress, and inherent inefficiencies—have historically imperiled product integrity and raised operational costs.

Alphinity is changing that equation. Evolving from ARTeSYN BioSolutions, a trailblazer in valve technology, Alphinity was founded with a singular mission: to transcend the limitations of conventional peristaltic, diaphragm, and centrifugal pumps. The company’s flagship innovation, PIXER® , is a singleuse component that seamlessly merges pumping and mixing functions while adhering to Pharma 4.0 standards.

“Innovation is our heartbeat at Alphinity. With PIXER® , we’re not just refining fluid management—we’re pioneering a new era in bioprocessing where precision, efficiency, and patient well-being converge to deliver transformative, life-saving therapies,” says Craig Vincze, Vice President & Co-Founder.

PIXER^®: A Breakthrough in Fluid Management

Pulsation at the pump outlet is a significant challenge for bioprocessing, disrupting electronic flow sensors and causing flow rate and pressure fluctuations. PIXER® eliminates these issues, ensuring smooth, precise fluid movement while optimizing efficiency. Designed for high-value biologics, it minimizes shear stress, operates at high pressures, and efficiently handles viscous fluids.

PIXER® ’s top inlet placement is a key mechanical innovation that allows direct positioning beneath a fluid reservoir, such as a bioreactor or retentate tank, in a tangential flow filtration (TFF) system. Unlike conventional pumps with side-positioned inlets, this vertical orientation leverages gravity for continuous fluid feed, enhancing suction efficiency and preventing air entrapment and cavitation—common issues in horizontal pump designs. This gravity-assisted design also reduces footprint and prevents tubing collapse under suction pressure, which is particularly beneficial for handling high-viscosity fluids or high flow rates.

PIXER® features a three, five, or seven-diaphragm radial pattern that minimizes hold-up volume, outperforming traditional linear diaphragm pumps. The design eliminates trapped air, facilitating quick purging while reducing shear stress on sensitive drug products such as monoclonal antibodies, proteins, and cells by ensuring uniform flooding of the diaphragms. This smooth, fluid entry results in a steady, low-pulsation outlet flow, preserving product integrity and enhancing performance with flow rates up to 200 liters per minute.

Another significant advantage is its multiple-outlet functionality. One outlet supports standard fluid transfer, while another recirculates fluid back to the vessel, maintaining homogeneity without requiring additional motors, shafts, or impellers. This streamlined approach reduces capital costs and eliminates the need for molded plastic covers for impeller rods, lowering footprint and quality control challenges. Many traditional systems require extra motors and impellers to ensure homogeneity, but PIXER® simplifies the process, reducing complexity and consumable costs.

Additionally, PIXER® delivers up to 50 percent more flow per RPM than competing diaphragm pumps, significantly improving energy efficiency and reducing power consumption in large-scale manufacturing. This efficiency translates to substantial energy savings, contributing to sustainability initiatives while maintaining peak performance. By enhancing fluid management with an innovative design, PIXER® sets a new standard in bioprocessing, ensuring reliable, cost-effective, and high-performance operation.

Powering Pharma 4.0 with Intelligent Integration

Alphinity’s pump features a strong mechanical design that embodies the concepts of Pharma 4.0 through innovative digitization and modular engineering. Its multiple inlets and outlets ensure flexible, efficient fluid transfer. At the same time, integrated cloud-native software—running on an edge computer— captures real-time data and delivers targeted insights for smarter decision-making.

This unified system streamlines operations like on-demand buffer dilution and cell media preparation, reducing space requirements and simplifying logistics. By enabling continuous, data-driven processing, these pumps minimize footprints and maximize throughput, all while ensuring consistent, high-quality outcomes.

Alphinity bridges hardware, software, and valve technologies to create a fully synchronized ecosystem. With embedded software engineers driving seamless integration, remote monitoring, and secure tech transfers, Alphinity leads the charge in smarter, faster, and more resilient biomanufacturing solutions.

Rethinking Buffer Management: Inline Buffer Dilution

Continuing its tradition of customer-centric innovation, Alphinity tackled the challenges of traditional buffer preparation faced by a leading biopharmaceutical manufacturer. Moving large buffer totes consumed valuable floor space and introduced logistical complexities that slowed production. Alphinity’s buffer dilution skid offered an immediate solution, enabling ondemand, point-of-use buffer production.

Building on this success, the manufacturer embraced Alphinity’s inline buffer dilution system, which integrates seamlessly with existing Water for Injection (WFI) infrastructure. The system dramatically reduced water usage and storage requirements by using six single-use bags filled with highly concentrated buffer stock (at 10x concentration). Operators select the desired buffer, specify the total volume, and initiate the process via an intuitive interface. The system then automatically verifies material availability and precisely mixes the concentrate with WFI to produce a ready-to-use 1x solution.

This innovation streamlines buffer preparation and simplifies downstream processes such as chromatography. Concentrated buffers are fed directly into the chromatography skid for realtime dilution and seamless transitions between different buffers, optimizing overall production efficiency, reducing energy consumption, and cutting operational costs.

Enabling Abundance in Medicine: Transforming Bioprocessing for a New Era

Alphinity’s mission to “enable abundance in medicine” transcends a simple tagline—it is a driving force shaped by personal experiences and a deep-seated commitment to expanding access to high-quality therapies. Recognizing the urgent need for both efficiency and precision in drug manufacturing, the company specializes in developing forward-thinking tools that empower process engineers to produce medicines more effectively. Central to this endeavor is enhancing critical hardware— beginning with the pump, the foundational element of any robust and repeatable biomanufacturing process.

With extensive industry expertise and a dedication to addressing real-world pain points, Alphinity approaches innovation with a transformative mindset. “Rather than iterating on existing designs, Alphinity rethinks fundamental principles— like developing a new alphabet before writing the book,” explains Vincze. Adopting this forward-thinking philosophy ensures that each solution goes beyond marginal improvements to deliver game-changing advancements aligned with the industry’s most pressing needs. Working hand in hand with customers from the earliest stages, Alphinity aims to provide process engineers with the right tools to shape the future of biomanufacturing.

As the next generation of treatments—from antibody-drug conjugates (ADCs) to cell and gene therapies—continues to advance, the necessity for robust, high-performance systems grows increasingly urgent. By redefining fluid management with PIXER® and integrating intelligent digital solutions, Alphinity establishes a new benchmark in bioprocessing.

The ultimate goal is straightforward yet profound: ensuring that life-saving therapies reach patients faster, more consistently, and at the highest possible quality standards.