How Automation Can Help in Genomic Analysis

Pharma Tech Outlook: Pharma Tech Magazine

How Automation Can Help in Genomic Analysis

By Pharma Tech Outlook | Friday, October 16, 2020

Automation in genomics helps increase the efficiency and speed of the DNA analysis. 

FREMONT, CA: The new advancement in medicine is increasing rapidly due to the latest developments in genome analysis. The DNA sequence analysis offers a better understanding of how genetic variation can lead to disease and develop new cures. The three crucial milestones achieved in the genome analysis are the Polymerase Chain Reaction (PCR), automated fluorescent DNA, and the invention of sequencing reactions that have allowed to increase efficiency and automate the maximum of the procedure necessary for DNA analysis. 

Nowadays, various instruments can be used to automate each step of the large-scale sequencing procedure like cloning or amplifying DNA, separating and detecting DNA fragments with fluorescent labels, isolating DNA, and preparing enzymatic sequencing reactions, and purifying DNA to achieve DNA sequence. The maximum of the large-scale sequencing facilities utilizes the consensus strategy that collaborates with a random shotgun phase and a directed finishing phase. Other centers utilize a whole-genome shotgun method like random fragments of total human genomic DNA that can be subscribed directly. High-throughput sequencing is used to generate random sequences that offer almost ten-fold coverage of the genome. There is also another process of sequencing every end of each BAC in the genomic library. Matching a finished sequence to several BAC ends allows selecting the net clone. 

Large-Scale Sequencing and Automation 

The automation used for the large-scale sequencing at various public and private genome centers can vary drastically in the machines and robots. The present automation system used by the sequencing centers can be considered evolutionary instead of revolutionary. The centers have selected discrete instruments to automate specific parts as per the requirement to meet the sequencing goals. These instruments also consist of in-house development and commercially accessible systems that can imitate the manual process stages. The system helps to improve dependability and throughput, along with reducing the expense.

Today, there is various type of systems available for genome automation. However, it is necessary to carefully choose each course so that it matches the processes and amount essential in that specific setting. Here are some of the automations systems available for different stages in the large-scale sequencing, DNA shearing devices, DNA template preparation systems, oligo synthesizers, thermal cyclers, and many more.

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